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A rapid method for chromatin structure analysis in the filamentous fungus Aspergillus nidulans
1Institut de Génétique et Microbiologie, URA CNRS D2225, Bâtiment 409, Université Paris-Sud, 91405 Orsay Cedex, France. rgonzalez@igmors.u-psud.fr
Nucleic Acids Research
|October 10, 1997
Abstract:
A rapid method for nuclease digestion of Aspergillus nidulans chromatin is described. It overcomes the need for nuclear purification or protoplast preparation. The method is valid for the analysis of the nucleosomal repeat length in bulk chromatin, and allows the analysis of nucleosome phasing at a specific locus.