Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacterial ghosts as multifunctional vaccine particles

M P Szostak1, H Mader, M Truppe

  • 1Institute of Microbiology and Genetics, University of Vienna, Austria.

Behring Institute Mitteilungen
|February 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence and molecular characterization of ascarid nematodes in cattle population from Malakand Division, Pakistan.

Helminthologia·2026
Same author

Regulation of chemicals demands assessment of risks rather than identification of hazards only.

Archives of toxicology·2026
Same author

The impact of targeted therapies on molecular alterations identified by an institutional molecular tumor board: an approach based on ESCAT classification.

ESMO real world data and digital oncology·2026
Same author

HPV circulating tumor DNA to monitor response to pembrolizumab and vorinostat combination in patients with advanced HPV-related squamous-cell carcinomas.

ESMO open·2025
Same author

Is Vitamin D Signaling Regulated by and Does It Regulate Circadian Rhythms?

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

ISUOG Basic Training program: contribution to improving theoretical sonographic knowledge.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025
Same journal

Clinical Experience with ALG.

Behring Institute Mitteilungen·2010
Same journal

Production of a Standardized Anti-Lymphocyte Globulin.

Behring Institute Mitteilungen·2010
Same journal

Immunosuppression and Neoplasia.

Behring Institute Mitteilungen·2010
Same journal

Molecular and Immunological Aspects of Host-Parasite Interactions. Symposium proceedings. Wurzburg, Germany, June 6-8, 1996.

Behring Institute Mitteilungen·1997
Same journal

The galactose-inhibitable surface lectin of Entamoeba histolytica, a possible candidate for a subunit vaccine to prevent amoebiasis.

Behring Institute Mitteilungen·1997
Same journal

Plasmodium vivax: epitope mapping of monoclonal antibodies against the N-terminal region of the merozoite surface protein 1.

Behring Institute Mitteilungen·1997
See all related articles

Bacterial ghosts, produced via PhiX174 gene E lysis, serve as non-living vaccines. These recombinant ghosts can carry foreign proteins, eliciting robust immune responses without denaturation.

Area of Science:

  • Microbiology
  • Immunology
  • Biotechnology

Background:

  • PhiX174 gene E induces bacterial lysis, forming transmembrane tunnels.
  • Bacterial ghosts (BGs) are non-living bacterial envelopes, useful as vaccine candidates.
  • Recombinant BGs can display foreign antigens, expanding their application.

Purpose of the Study:

  • To detail the production and applications of bacterial ghosts as vaccine carriers.
  • To highlight the advantages of BGs over traditional inactivated vaccines.
  • To demonstrate the immunogenicity of antigens presented by BGs.

Main Methods:

  • Expression of PhiX174 gene E in Gram-negative bacteria for lysis.
  • Production of BGs from various bacterial species.
  • Insertion of foreign proteins into BGs using anchor sequences.

Related Experiment Videos

  • Export of proteins into periplasmic space or expression as S-layer proteins.
  • Application of BGs in experimental animals (oral, aerogenic, parenteral).

    • Main Results:

    • Successful production of BGs from diverse bacterial species.
    • Demonstrated insertion and display of foreign proteins in BGs.
    • Induction of specific humoral and cellular immune responses in animals.
    • Evidence of protective mucosal immunity following BG administration.
    • Antigen size is not a limitation for insertion into BGs.

    Conclusions:

    • Bacterial ghosts are effective, non-denaturing vaccine platforms.
    • Recombinant BGs offer versatile antigen presentation for enhanced immunogenicity.
    • BGs represent a promising alternative to conventional inactivated vaccines.