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Related Experiment Videos

Translational attenuation mediated by an mRNA intron

R E Chapman1, P Walter

  • 1Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco 94143-0448, USA.

Current Biology : CB
|February 28, 1998
PubMed
Summary
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The HAC1 mRNA intron prevents translation, even when the mRNA is in the cytoplasm. This finding reveals a novel mechanism for regulating gene expression via mRNA introns.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Expression Regulation

Background:

  • The unfolded protein response (UPR) is activated by endoplasmic reticulum (ER) stress.
  • UPR activation involves regulated splicing of HAC1 mRNA to produce the Hac1p transcription factor.
  • Uninduced HAC1 mRNA (HAC1u) is stable but does not produce detectable Hac1p.

Purpose of the Study:

  • To investigate why unspliced HAC1 mRNA (HAC1u) does not produce Hac1p.
  • To determine the role of the HAC1 intron in translation regulation.

Main Methods:

  • Tracking HAC1u mRNA localization and polyribosome association.
  • Assessing Hac1p production from unspliced and spliced HAC1 mRNA.
  • Analyzing Hac1p modification and activity.

Related Experiment Videos

  • Transplanting the HAC1 intron into a heterologous mRNA system.

    • Main Results:

    • HAC1u mRNA is exported to the cytosol and associates with polyribosomes.
    • The HAC1 intron prevents translation of HAC1u mRNA.
    • Hac1p produced from unspliced mRNA (Hac1pu) is less active than Hac1pi.
    • The HAC1 intron alone can attenuate translation of other mRNAs.

    Conclusions:

    • The HAC1 mRNA intron is essential and sufficient to block translation of polyribosome-associated mRNA.
    • This study identifies a novel mechanism of translational attenuation mediated by an mRNA intron.