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Published on: August 2, 2011
Detection of four serotypes of avian adenovirus in New Zealand
Abstract:
Twenty agents with adenovirus morphology were recovered from New Zealand domestic hens by means of chick kidney cell cultures. All the agents gave distinct cytopathic effects (CPE) in cell cultures, with two different types of CPE observed. On the basis of neutralization tests, the twenty agents were assigned to four distinct serological groups. Physicochemical tests on a "prototype" strain from each serological group confirmed that these agents are adenoviruses. The four prototype strains recovered in New Zealand are related to established overseas strains. One strain is serologically related to agents that cause IBH.
Insights
Researchers identified twenty adenovirus agents in New Zealand hens. These agents, causing distinct cell damage, were grouped into four types and confirmed as adenoviruses, with one linked to infectious bronchitis virus (IBH).
Area of Science:
- Veterinary Virology
- Avian Pathology
- Molecular Biology
Background:
- Adenoviruses are significant pathogens in poultry, causing various diseases.
- Characterizing novel viral strains is crucial for disease prevention and control in domestic animals.
Purpose of the Study:
- To isolate and characterize adenovirus agents from domestic hens in New Zealand.
- To determine the serological and physicochemical properties of these agents.
- To assess their relationship to known adenovirus strains and diseases like infectious bursal hyperplastic disease (IBH).
Main Methods:
- Isolation of viral agents using chick kidney cell cultures.
- Observation and classification of distinct cytopathic effects (CPE).
- Serological grouping via neutralization tests.
- Physicochemical characterization of prototype strains.
Main Results:
- Twenty agents with adenovirus morphology were successfully recovered.
- Two distinct types of cytopathic effects were observed in cell cultures.
- The agents were classified into four distinct serological groups.
- Physicochemical tests confirmed the agents as adenoviruses.
- Four New Zealand strains showed relatedness to established overseas strains.
- One strain exhibited serological relation to agents causing IBH.
Conclusions:
- The study successfully identified and characterized novel adenovirus strains in New Zealand domestic hens.
- These findings contribute to understanding adenovirus diversity and epidemiology in poultry.
- The identification of a strain related to IBH-causing agents highlights potential disease risks.
