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Related Experiment Videos

Mice lacking factor VII develop normally but suffer fatal perinatal bleeding

E D Rosen1, J C Chan, E Idusogie

  • 1Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium.

Nature
|December 31, 1997
PubMed
Summary

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Blood coagulation factor VII (FVII) is essential for blood clotting. Surprisingly, FVII-deficient embryos survive, indicating tissue factor (TF) has roles beyond initiating blood coagulation during development.

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Hematology

Background:

  • Blood coagulation is initiated by factor VII (FVII) binding to tissue factor (TF).
  • TF-deficient embryos exhibit defective circulation and embryonic lethality around 9.5 days of gestation.
  • Given FVII is the sole known TF ligand, FVII-deficient embryos were predicted to share a similar phenotype.

Purpose of the Study:

  • To investigate the in vivo role of FVII during embryonic development.
  • To determine if FVII deficiency phenocopies TF deficiency in embryonic development.
  • To elucidate the function of TF beyond fibrin formation in embryogenesis.

Main Methods:

  • Generation and analysis of FVII-deficient (FVII-/-) mice.
  • Phenotypic assessment of FVII-/- embryos during gestation.

Related Experiment Videos

  • Evaluation of maternal-fetal transfer of FVII and TF-FVII-initiated fibrin formation at embryonic day 9.5.

    • Main Results:

    • FVII-deficient embryos developed normally without apparent defects in circulation.
    • FVII-/- mice survived until perinatal stages but succumbed due to fatal hemorrhaging.
    • Maternal-fetal transfer of FVII was undetectable at embryonic day 9.5, and embryo survival was independent of TF-FVII-initiated fibrin formation.

    Conclusions:

    • The normal development of FVII-deficient embryos contrasts with the embryonic lethality of TF-deficient embryos.
    • Embryonic survival is not solely dependent on TF-FVII-initiated fibrin formation.
    • Tissue factor plays a critical role in embryogenesis independent of its function in initiating blood coagulation.