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Nonaqueous capillary electrophoresis in pharmaceutical analysis
I Bjørnsdottir1, J Tjørnelund, S H Hansen
1Department of Analytical and Pharmaceutical Chemistry, Royal Danish School of Pharmacy, Copenhagen, Denmark.
Journal of Capillary Electrophoresis
|March 1, 1996
Summary
Nonaqueous media offer high selectivity for capillary electrophoresis (CE) separations of closely related compounds without additives. This study explores solvent properties and compares aqueous vs. nonaqueous methods for drug impurity analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Achieving high selectivity for similar compounds can be challenging in aqueous media.
Purpose of the Study:
- To investigate the utility of nonaqueous media for capillary electrophoresis (CE) separations.
- To compare the separation performance of nonaqueous and aqueous media for small compounds.
- To explore the application of nonaqueous CE in drug impurity testing.
Main Methods:
- Electrophoretic separations were conducted in various nonaqueous media.
- Physicochemical properties of selected solvents were analyzed.
- Separations in nonaqueous media were compared to those in aqueous media.
- Drug impurity testing was performed using nonaqueous CE.
Main Results:
- Nonaqueous media enable high selectivity for closely related compounds without additives.
- Physicochemical solvent parameters significantly influence separation outcomes.
- Nonaqueous CE demonstrated effectiveness for separating compounds with similar charge-to-mass ratios.
- Successful application of nonaqueous CE for drug impurity analysis was demonstrated.
Conclusions:
- Nonaqueous media are advantageous for achieving high selectivity in CE.
- Understanding solvent properties is crucial for optimizing nonaqueous CE separations.
- Nonaqueous CE offers a viable alternative for sensitive drug impurity testing.