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A highly stable methyl cellulose coating for capillary electrophoresis
J L Liao1, J Abramson, S Hjertén
1Department of Biochemistry, University of Uppsala, Sweden.
Journal of Capillary Electrophoresis
|July 1, 1995
Summary
A new capillary coating made from methyl cellulose and silane provides a stable surface that significantly reduces electroendosmotic flow (EOF) and sample adsorption for extended periods.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Capillary electrophoresis (CE) relies on stable capillary surfaces to minimize electroendosmotic flow (EOF) and sample adsorption.
- Existing coatings often lack long-term stability or require complex preparation.
Purpose of the Study:
- To develop a highly stable capillary coating for CE applications.
- To covalently couple methyl cellulose to a silane-modified fused silica surface.
- To evaluate the coating's performance in suppressing EOF and sample adsorption.
Main Methods:
- Covalent coupling of methyl cellulose hydroxyl groups to alpha-glycidoxypropyltrimethoxysilane on fused silica tubing.
- Testing coating stability using isoelectric focusing and free zone electrophoresis.
- Assessing EOF suppression and sample adsorption over extended periods and extreme pH conditions.
Main Results:
- A highly stable capillary coating was successfully created.
- The coating significantly suppressed electroendosmotic flow (EOF) for weeks to months.
- The coating demonstrated excellent stability, withstanding 135 runs and 30 days in 0.01 M NaOH.
- Sample adsorption onto the capillary wall was minimized.
Conclusions:
- The developed capillary coating offers exceptional stability and performance for CE.
- This method provides a robust and easy-to-perform procedure for preparing stable CE capillaries.
- The coating effectively minimizes EOF and sample adsorption, enhancing analytical reproducibility.