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Immunological identification of a basic homologue to acidic virus-inducible cucumber peroxidase
1Laboratory of Molecular Biology and Virology, Complex Research Institute of Viticulture and Enology, Bratislava, Slovak Republic.
Abstract:
Eight basic, fast-moving pathogenesis-related peroxidase PR-PRX isoenzymes were found to accumulate in cotyledons of cucumber (Cucumis sativus L., cv.Laura) reacting hypersensitively to tobacco necrosis virus (TNV). These isozymes were provisionally designated a, b, c, d, e, f, and g in the order of decreasing electrophoretic mobility in native cathodic polyacrylamide gels. Besides the differential mode of compartmentalization, some of these isoenzymes serologically cross-reacted with the antiserum prepared against the most prominent and best characterized cucumber PR-protein, an anionic PR-PRX. The immunoblot analysis confirmed that, by analogy with the tobacco PR-proteins 1, acidic and basic PR-PRXs also belong to a family of differently charged isomers.