Related Experiment Video
Updated: Jul 13, 2026
09:40
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Cloning and functional analysis of rat cyclin D2 promoter: multiple prolactin-responsive elements
M Yang1, Y Hosokawa, Y Hu
1Department of Biochemistry, Mie University Faculty of Medicine, Japan.
Biochemistry and Molecular Biology International
|February 7, 1998
Summary
The rat cyclin D2 gene promoter
Area of Science:
- Molecular Biology
- Gene Regulation
- Cell Cycle Research
Background:
- Cyclin D2 is a key regulator of cell cycle progression.
- Understanding gene regulation is crucial for cell biology.
- Prolactin signaling plays a role in cell proliferation.
Purpose of the Study:
- To investigate the promoter region of the rat cyclin D2 gene.
- To identify regulatory elements controlling cyclin D2 expression.
- To understand the role of prolactin in cyclin D2 gene induction.
Main Methods:
- Cloning of the rat cyclin D2 gene promoter region.
- DNA sequence analysis of the promoter.
- DNA mobility shift assay using nuclear extracts from prolactin-stimulated Nb2 cells.
Main Results:
- The rat cyclin D2 gene promoter is not TATA-box dependent.
- Multiple transcription factors are involved in cyclin D2 gene regulation.
- Prolactin induces transcription factors at an immediate-early stage of the cell cycle.
Conclusions:
- Rat cyclin D2 gene expression is regulated by prolactin-induced transcription factors.
- The TATA-box is not essential for cyclin D2 gene expression in these cells.
- Prolactin acts at an early stage to stimulate cyclin D2 gene expression, influencing cell cycle progression.
Related Concept Videos
01:58Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
02:40The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.