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09:40
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Cloning and functional analysis of rat cyclin D2 promoter: multiple prolactin-responsive elements
M Yang1, Y Hosokawa, Y Hu
1Department of Biochemistry, Mie University Faculty of Medicine, Japan.
Biochemistry and Molecular Biology International
|February 7, 1998
Abstract:
Structure and function of the promoter region of rat cyclin D2 gene were investigated by cloning, sequence analysis and DNA mobility shift assay using nuclear extracts of prolactin-stimulated Nb2 cells. The results show that the expression of rat cyclin D2 gene is not dependent on the TATA-box but is stimulated by multiple transcription factors induced by prolactin at an immediate-early stage of the cell cycle.
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