Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue-engineered heart valve leaflets: does cell origin affect outcome?

T Shinoka1, D Shum-Tim, P X Ma

  • 1Department of Cardiovascular Surgery, Children's Hospital, Boston, Mass 02115, USA.

Circulation
|December 31, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diverse Properties of Electron Forbush Decreases Revealed by the Dark Matter Particle Explorer.

Physical review letters·2026
Same author

Measurement of the Cosmic Ray Helium Energy Spectrum from 70 GeV to 80 TeV with the DAMPE Space Mission.

Physical review letters·2021
Same author

Functional Assessment of Pluripotent and Mesenchymal Stem Cell Derived Secretome in Heart Disease.

Annals of stem cell research·2020
Same author

Measurement of the cosmic ray proton spectrum from 40 GeV to 100 TeV with the DAMPE satellite.

Science advances·2019
Same author

Role of the partner/spouse in melanoma discovery and related health behaviours and practices.

The British journal of dermatology·2019
Same author

Autonomous Robotic Intracardiac Catheter Navigation Using Haptic Vision.

Science robotics·2019
Same journal

Eugene Braunwald, MD, 1929-2026.

Circulation·2026
Same journal

AHA/ACC/ESC/WHF Expert Consensus Document: Second Universal Definition of Heart Failure (2026).

Circulation·2026
Same journal

Advancing Quality in the Evaluation, Surveillance, and Management of Aortic Stenosis: A Report From the AHA Target: AS Registry.

Circulation·2026
Same journal

Heart Failure Occurring in the Perinatal Period: A Scientific Statement From the American Heart Association.

Circulation·2026
Same journal

Correction to: 2026 ACC/AHA/AACVPR/ABC/ACPM/ADA/AGS/APhA/ASPC/NLA/PCNA Guideline on the Management of Dyslipidemia: A Report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines.

Circulation·2026
Same journal

Correction to: The Natural History of Massive Left Ventricular Hypertrophy in Pediatric Hypertrophic Cardiomyopathy: A Multiregistry Analysis.

Circulation·2026
See all related articles

Tissue-engineered valve leaflets (TEL) derived from arterial myofibroblasts showed better growth and structure compared to dermal fibroblasts in a lamb model. Arterial cells are preferable for creating functional autologous tissue-engineered valve leaflets.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cardiovascular Research

Background:

  • Previous success in creating autologous tissue-engineered valve leaflets (TEL) for pulmonary valve replacement in lambs.
  • The optimal cell source for these engineered leaflets remains undetermined.

Purpose of the Study:

  • To compare dermal fibroblasts with arterial wall myofibroblasts as the cell origin for tissue-engineered valve leaflet constructs.
  • To evaluate the functional and morphological development of these autologous constructs in vivo.

Main Methods:

  • Isolation and in vitro expansion of endothelial cells and fibroblasts from ovine dermal and arterial sources.
  • Seeding of cells onto biodegradable polyglactin and polyglycolic acid polymer scaffolds.
  • Implantation of autologous tissue-engineered leaflets to replace a pulmonary valve leaflet in juvenile lambs.

Related Experiment Videos

  • Histological, biochemical, and biomechanical analysis of explanted leaflets after 8-10 weeks.

    • Main Results:

    • All implanted tissue-engineered leaflets persisted in the pulmonary position, with complete polymer degradation.
    • Arterial-derived leaflets (Group A) exhibited a significantly higher growth index than dermal-derived leaflets (Group D).
    • Histology revealed more abundant elastic fibers in arterial-derived leaflets; dermal leaflets showed macroscopic thickening and contraction.

    Conclusions:

    • Autologous tissue-engineered leaflets derived from vascular fibroblasts integrate and develop similarly to native valve leaflets.
    • Arterial myofibroblasts are a more suitable cell source than dermal fibroblasts for creating tissue-engineered valve leaflets under current conditions.