Purification of human blood clotting factor X by Blue Dextran agarose affinity chromatography

Blue Dextran 2000 coupled covalently to agarose has been used as an affinity column for the rapid separation of human blood clotting Factor X. Factor X has been isolated with approximately 2000-fold purification from human citrated plasma and shows single-component behavior by sodium dodecyl sulfate gel electrophoresis. Affinity columns prepared with Blue Dextran chromophore (Cibacron blue) derivatized to agarose or Sepharose gave negative results. These studies have shown that Blue Dextran agarose possesses unique biospecific and nonbiospecific properties, both of which are essential to achieve resolution of Factor X from other vitamin K-dependent blood clotting factors.