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[Monocellular quantitative study on the adhesive ability of eosinophils in the course of eosinophils activation]
1Department of Respiration, Dapin Hospital, Third Medical University, Chongqing.
Objective:
To observe the dynamic changes of eosinophils (EOS) adhesive ability in the course of eosinophils activation.
Methods:
The adhesive ability EOS to vascular endothelial cell (EC) was measured by the use of a microminpulation technique.
Results:
The critical separation stress (Sc) between rest EOS and EC was 0.61 +/- 0.07kPa. Treatment of EOS with platelet activaty factor (PAF) caused a significant increase in Sc. The Sc was 4.1 +/- 0.6kPa within 30 minutes of EOS exposure to PAF, and had no tendency to increase continuously by exposure time prolonged.
Conclusions:
The increase of EOS adhesive ability belongs to a kind of rapid response while EOS is activated. The increase could be an important basis of EOS adhesion to EC and EOS entry into bronchial mucosa.