[Experimental research on the distribution of insulin in condyle during mandibular functional protrusion in growing...

The purpose of this study is to evaluate insulin change in condylar cartilage during functional advancement of rat mandible by immunohistochemical technique (ABC method) in order to provide further information about the role of insulin in orthopedics and the relationship between insulin and proliferation of condylar cartilage. Staining gradient of insulin is observed in condylar cartilage, with the stain deepest in the geriminal layer, medial in the transitional, least in the maturational layer. The posterior region of condylar cartilage in the experimental groups is shown to have thickened significantly as compared with the control groups, especially the germinal layer. Stained-positive cells exhibit deeper stain and higher percentage. The stain in the other layers is also more obvious than that in the control groups. The results suggest: Insulin level in condyle changes with the different cartilage layers. Insulin distribution appears higher when condylar chondrocytes proliferate rapidly and lower when they differentiate actively. Functional orthopedics may stimulate the growth of condylar cartilage and raise the insulin level. The demonstrates that insulin correlates with hyperplasia of conylar chondrocytes.