[Different proteins in NRCF (nerve regeneration conditioned fluid) from the proximal and distal nerve stump]

80 SD rats were randomly divided into four groups of 20 each; electrostimulation of myeloneure (Group N); electrostimulation of the denervated muscle (Group M); Group C1; and Goup C2. The left lateral sciatic nerve of rats was exercised 2mm in length, and the distal nerve stump of the goup C2 and M and proximal nerve stamp of the Group C1 and N were each sulured into the silcon chamber with the opposile end ligated so that a 10mm was left between the stump and the blind end of chamber. At 3rd, 7th, 14th, 21th and 28th day after nerve transection and tube implantation, the fluid in the silicon tube was aspirated. The samples of fluid were spun and 4 microliters aliguots were taken from the super ratant. Then subjected to the aliquots were taken by phase system and assayed by Gel scan system. The results showed that the Group C2 and M had highly increase in the protein amount. Than that of group C1 and N in the range of 6-10 KD molecular weight. In 29-47 KD molecular weight range, the variety of protein molecular weight in aliguots of the Group N had and obvious increase compared with the group C1. In 16-29 KD, the protein amount in the Group M was more than that in the Group C2.