[Studies of murine ectoplacental cone cells interaction with laminin]

Implantation of the mouse embryo is dependent on the interactions between the trophoblast cells and the surrounding uterine environment. The initial invasion by primary trophoblast stimulates the uterine stromal fibroblasts differentiate into decidual cells, which deposit a pericellular matrix consisting of LN, FN and Col IV. The secondary trophoblast giant cells (TGCs) from ectoplacental cone (EPC) invade the decidua to form the fetal portion of the placenta. We used synthetic peptides cyclic YIGSR (cYIGSR) and RGDS to study the mechanisms of EPC cells interaction with LN. The results indicated that cYIGSR and RGDS promoted EPC attachment and had synergistic effect, and cYIGSR also promoted EPC outgrowth and secondary TGCs migration. LN supported EPC attachment and outgrowth, as well as secondary TGCs migration from EPC. Biologically active domains RGD of LN A chain and YIGSR of LN B1 chain participated synergistically in EPC attachment, outgrowth, as well as secondary TGCs migration. Since synthetic peptides cYIGSR and RGDS can't competitively inhibit EPC attachment with LN completely, there must be other binding sites involved in the interaction.