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Related Experiment Videos

Apoptosis in sea urchin embryos

M C Roccheri1, G Barbata, F Cardinale

  • 1Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, Università di Palermo, Italy. dipbio@mbox.unipa.it

Biochemical and Biophysical Research Communications
|December 6, 1997
PubMed
Summary

TPA plus heat treatment induces apoptosis in Paracentrotus embryos. Untreated embryos also exhibit spontaneous apoptosis during the early pluteus stage, particularly in arm and intestine tissues.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Marine Biology

Background:

  • Apoptosis, or programmed cell death, is crucial for normal development.
  • Understanding apoptosis in marine invertebrate embryos provides insights into conserved biological processes.

Purpose of the Study:

  • To investigate the induction of apoptosis in Paracentrotus embryos by specific treatments.
  • To observe spontaneous apoptosis in untreated Paracentrotus embryos during development.

Main Methods:

  • DNA electrophoresis analysis
  • Morphological observations
  • Terminal deoxynucleotidyl transferase (TdT) in situ reaction

Main Results:

  • Treatment with TPA plus heat significantly induced an apoptotic reaction in Paracentrotus embryos.

Related Experiment Videos

  • Untreated embryos displayed spontaneous apoptosis, predominantly in the arm and intestine regions at the early pluteus stage.

    • Conclusions:

    • TPA plus heat is an effective inducer of apoptosis in these marine embryos.
    • Spontaneous apoptosis in specific embryonic tissues suggests a role in normal developmental processes or tissue remodeling.