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Bcl-6 and lymphoproliferative disorders

G Y Michaud1, R D Gascoyne, B K McNeil

  • 1British Columbia Cancer Agency, Department of Pathology, University of British Columbia, Canada.

Leukemia & Lymphoma
|August 1, 1997
PubMed
Summary

Structural chromosomal abnormalities at 3q27, near the bcl-6 gene, are linked to various B-cell lymphomas. These rearrangements, often involving immunoglobulin genes, may contribute to lymphomagenesis.

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Area of Science:

  • Genetics
  • Oncology
  • Molecular Biology

Background:

  • Lymphoproliferative disorders encompass a range of B-cell malignancies.
  • Chromosomal abnormalities play a significant role in lymphomagenesis.
  • The bcl-6 gene, located at 3q27, is implicated in B-cell development.

Purpose of the Study:

  • To investigate the frequency and spectrum of 3q27 structural chromosomal abnormalities in lymphoproliferative disorders.
  • To determine the association of these abnormalities with specific lymphoma subtypes.
  • To explore the role of bcl-6 gene deregulation in lymphomagenesis.

Main Methods:

  • Karyotypic analysis of 37 cases with lymphoproliferative disorders.
  • Break-apart fluorescence in situ hybridization (FISH) for bcl-6 rearrangements.

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  • Immunohistochemistry to assess bcl-6 protein expression.

    • Main Results:

    • 37 cases exhibited 3q27 structural chromosomal abnormalities.
    • Abnormalities were most frequent in follicular lymphomas without t(14;18) and diffuse large B-cell lymphomas.
    • Most rearrangements involved immunoglobulin heavy or light chain genes.
    • Molecular rearrangement of bcl-6 was detected in a subset of cases.

    Conclusions:

    • 3q27 structural abnormalities are recurrent in B-cell lymphomas, particularly follicular and diffuse large B-cell lymphomas.
    • Translocations involving immunoglobulin genes are the primary mechanism for 3q27 rearrangements.
    • Transcriptional deregulation of bcl-6 may contribute to lymphomagenesis.