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Positive selection of CD56+ lymphocytes by magnetic cell sorting
A Geiselhart1, S Neu, F Buchholz
1University Children's Hospital, Department of Hematology/Oncology, Tübingen, Germany.
Natural Immunity
|January 1, 1996
Summary
A novel method effectively isolates CD56+ lymphocytes using magnetic microbeads, achieving high purity. This technique preserves natural killer cell activity and proliferative responses for potential immunotherapy applications.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- CD56+ lymphocytes, crucial for immune responses, require efficient isolation methods for research and therapy.
- Existing isolation techniques may impact cell function, limiting their clinical utility.
Purpose of the Study:
- To develop and validate a new method for isolating CD56+ lymphocytes from peripheral blood mononuclear cells.
- To assess the purity, recovery, and functional integrity of isolated CD56+ lymphocytes.
Main Methods:
- Utilized magnetic microbeads conjugated to goat antimouse antiserum and a murine monoclonal anti-CD56 antibody for target cell labeling.
- Employed a magnetic cell sorter for the isolation of labeled CD56+ cells.
Main Results:
- Achieved high purity of CD56+ cells (98.4 +/- 1%) with a recovery rate of 57.2 +/- 9%.
- Demonstrated that the isolation procedure and magnetic microbeads did not impair natural killer cell activity or interleukin-2-induced proliferation.
Conclusions:
- The described magnetic bead-based method provides a highly pure and functional population of CD56+ lymphocytes.
- This technique is a valuable tool for CD56+ cell characterization and can be adapted for clinical applications, including adoptive immunotherapy.