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Experience-dependent modifications in MAP2 phosphorylation in rat olfactory bulb

B D Philpot1, J H Lim, S Halpain

  • 1University of Virginia, Department of Psychology, Charlottesville, Virginia 22903, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|January 10, 1998
PubMed
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Experience-dependent changes in the olfactory bulb involve Microtubule-associated protein 2 (MAP2) phosphorylation. Olfactory restriction reduces MAP2 phosphorylation on Ser136, indicating activity-dependent regulation of this crucial cytoskeletal protein.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Microtubule-associated protein 2 (MAP2) is a neuron-specific cytoskeletal protein vital for dendritic structure.
  • MAP2's regulation of microtubule stability is phosphorylation-dependent.
  • Experience-driven plasticity in the olfactory bulb is a key area of developmental neuroscience research.

Purpose of the Study:

  • To investigate experience-dependent changes in MAP2 expression and phosphorylation during olfactory bulb development.
  • To determine if olfactory activity influences MAP2 phosphorylation state using immunocytochemistry.

Main Methods:

  • Utilized phosphoepitope-specific and phosphorylation state-independent antibodies for MAP2.
  • Employed unilateral naris closure in developing rats to block olfactory input.

Related Experiment Videos

  • Quantified MAP2 immunoreactivity in the olfactory bulb granule cell layer.

    • Main Results:

    • Total MAP2 expression peaked by postnatal day 20 (P20).
    • Phosphorylation-independent MAP2 staining was unaffected by naris closure.
    • Olfactory restriction significantly reduced phosphorylation of MAP2 on Ser136 (detected by AP18 antibody), with the effect dependent on deprivation duration.

    Conclusions:

    • Olfactory bulb MAP2 phosphorylation on Ser136 is regulated by sensory experience.
    • The observed reduction in MAP2 phosphorylation suggests an activity-dependent stimulation mechanism.
    • This phosphorylation shift is dependent on the duration of olfactory input, not solely its onset time.