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Related Experiment Videos

Partial purification of human renin substrate

V Schioler, M Damkjaer, M Nielsen

    European Journal of Clinical Investigation
    |June 21, 1976
    PubMed
    Summary

    Researchers developed a new method to purify renin substrate from human plasma, achieving a 57% yield and twenty-fold purification. This method provides a highly pure renin substrate for further research and applications.

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    Area of Science:

    • Biochemistry
    • Proteomics

    Background:

    • Renin substrate is crucial for the renin-angiotensin system.
    • Accurate measurement requires highly purified substrate.

    Purpose of the Study:

    • To develop an efficient purification method for human plasma renin substrate.
    • To characterize the purity and specific activity of the prepared renin substrate.

    Main Methods:

    • Two-step purification of renin substrate from pooled human plasma.
    • Enzymatic degradation with human renin to determine specific activity.
    • Affinity chromatography for enhanced purification in pilot studies.

    Main Results:

    • Achieved 57% overall yield and twenty-fold purification with the two-step method.
    • Specific activity of 2 mug angiotensin I per mg protein.
    • Pilot studies with affinity chromatography yielded 37% and hundred-fold purification (10 mug angiotensin I/mg protein).

    Conclusions:

    • The described method efficiently purifies human plasma renin substrate.
    • The purified substrate is suitable for biochemical assays due to low endogenous activity and absence of angiotensinase.
    • Affinity chromatography further enhances purity and specific activity.

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