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Praja1, a novel gene encoding a RING-H2 motif in mouse development
L Mishra1, R E Tully, S P Monga
1Laboratory of Developmental Molecular Biology, VA Medical Center, Washington, DC, USA.
Oncogene
|December 11, 1997
Summary
Researchers identified Praja1, a novel gene crucial for early mouse liver development. This gene, located on the X chromosome, may play a role in protein interactions and cellular transport pathways.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- Early mouse liver development involves complex genetic regulation.
- The Drosophila melanogaster gene goliath (gl) influences mesodermal cell fate.
- Identifying novel genes is key to understanding developmental processes.
Purpose of the Study:
- To identify genes involved in early mouse liver development.
- To characterize the Praja1 gene and its potential function.
- To investigate the role of Praja1 in cellular processes.
Main Methods:
- Gene cloning and sequencing.
- Gene localization using the Jackson Laboratory BSS panel.
- Northern blot analysis of Praja1 transcripts.
- In vitro transcription/translation and immunohistochemical staining.
Main Results:
- Praja1, a 2.1 kb gene encoding a 396 amino acid ORF with a COOH-terminal RING-H2 domain, was isolated.
- Praja1 was localized to chromosome X at 36 cM, near Xist, and is a candidate for mouse sex-linked sideroblastic anemia (sla).
- Praja1 exhibits three transcripts in adult mouse tissues and developing embryos; its product has an Mr of 59 kD and localizes to the cytoplasm of hepatocyte-like cells.
Conclusions:
- Praja1 is a novel gene implicated in early mouse liver development.
- The presence of a RING-H2 domain suggests Praja1 mediates protein-protein interactions, potentially in protein sorting or transport.
- Praja1's similarity to rat Neurodap1 further supports its role in cellular transport pathways.