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Intercellular adhesion molecule-1 mediated interactions and leucocyte infiltration in IgA nephropathy
P Arrizabalaga1, M Solé, I L Quintó
1Service of Nephrology, Hospital Clínic i Provincial, University of Barcelona, Spain.
Summary
In IgA nephropathy (IgAN), increased intercellular adhesion molecule-1 (ICAM-1) expression on kidney tubules correlates with leucocyte infiltration. This suggests ICAM-1 plays a role in leucocyte adhesion and kidney damage in IgAN.
Area of Science:
- Nephrology
- Immunology
- Pathology
Background:
- Mononuclear leucocytes are implicated in IgA nephropathy (IgAN).
- Leucocyte recruitment in the kidney involves adhesion molecule interactions.
- Intercellular adhesion molecule-1 (ICAM-1) is a key adhesion molecule.
Purpose of the Study:
- To investigate the relationship between leucocyte infiltration and ICAM-1 expression in IgAN kidneys.
- To identify specific leucocyte subsets involved in ICAM-1 expression in IgAN.
Main Methods:
- Renal biopsy sections from 48 IgAN patients were analyzed.
- Immunohistochemistry with monoclonal antibodies was used to identify leucocytes (CD45, CD18, CD14, CD3, CD4, CD8) and ICAM-1 (CD54).
- Patients were stratified based on interstitial cellular infiltration severity.
Main Results:
- Abnormal ICAM-1 expression was found on proximal tubule epithelium in IgAN with moderate to severe interstitial infiltration (2+ to 4+).
- Tubular ICAM-1 expression significantly correlated with interstitial leucocytes, including CD45+, CD14+, and CD3+ cells.
- Interstitial ICAM-1+ cells correlated with CD18+ leucocytes, and tubular ICAM-1 expression correlated with glomerular CD45+, CD3+, and CD8+ leucocytes.
Conclusions:
- Tubular and interstitial ICAM-1+ cells likely participate in leucocyte adhesion in IgAN.
- Interstitial T-cells and macrophages, along with glomerular CD8+ T-cells, may induce tubular ICAM-1 expression in IgAN.