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Related Experiment Videos

Aggregation-promoting factor in human vaginal Lactobacillus strains

V Kmet1, F Lucchini

  • 1Institute of Animal Physiology, Slovak Academy of Sciences, Kosice. kmetv@linux1.saske.sk

FEMS Immunology and Medical Microbiology
|December 12, 1997
PubMed
Summary

Lactobacillus strains from healthy women exhibit cell surface hydrophobicity and auto-aggregation. An aggregation promoting factor (APF) in Lactobacillus mediates this, potentially inhibiting uropathogenic Escherichia coli.

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Area of Science:

  • Microbiology
  • Bacterial interactions
  • Human microbiome

Background:

  • Lactobacillus species are key commensal bacteria in the human vaginal microbiota.
  • Uropathogenic Escherichia coli (UPEC) are a common cause of urinary tract infections.
  • Understanding interactions between vaginal Lactobacillus and UPEC is crucial for preventing infections.

Purpose of the Study:

  • To investigate auto-aggregation and cell surface hydrophobicity in vaginal Lactobacillus strains.
  • To identify mechanisms of bacterial aggregation, including the role of aggregation promoting factor (APF).
  • To explore the coaggregation potential between Lactobacillus and UPEC.

Main Methods:

  • Isolation and characterization of Lactobacillus strains from healthy women's vaginal samples.

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  • Assessment of auto-aggregation and cell surface hydrophobicity.
  • DNA hybridization to confirm the presence of the APF gene.
  • Coaggregation assays with uropathogenic Escherichia coli strains.

    • Main Results:

    • Twelve homofermentative Lactobacillus strains showed correlated cell surface hydrophobicity and auto-aggregative activity.
    • Aggregation promoting factor (APF) was identified in Lactobacillus sp. strain HV 142, confirmed by DNA hybridization.
    • Auto-aggregative vaginal lactobacilli coaggregated with P-fimbriated uropathogenic Escherichia coli.
    • One Lactobacillus strain (HV 389) aggregated with tested E. coli strains.

    Conclusions:

    • Cell surface hydrophobicity and auto-aggregation are significant characteristics of vaginal Lactobacillus.
    • Aggregation promoting factor (APF) is a key mechanism for Lactobacillus auto-aggregation.
    • APF-producing lactobacilli may play a role in inhibiting uropathogenic E. coli colonization through coaggregation.