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Dentin sialoprotein (DSP) transcripts: developmentally-sustained expression in odontoblasts and transient expression

H H Ritchie1, J E Berry, M J Somerman

  • 1Department of Basic Sciences, University of Texas-Houston Health Science Center, Dental Branch, 77030, USA. helena-ritchie@uiowa.edu

European Journal of Oral Sciences
|December 12, 1997
PubMed
Summary

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Dentin sialoprotein (DSP) mRNA is primarily found in odontoblasts during tooth development. Transient DSP expression also occurs in pre-ameloblasts, suggesting a role in epithelial-mesenchymal interactions crucial for tooth formation.

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Oral Biology

Background:

  • Dentin sialoprotein (DSP) is a glycoprotein traditionally considered exclusive to dentin.
  • Understanding DSP expression patterns is key to elucidating dentinogenesis mechanisms.

Purpose of the Study:

  • To investigate the localization and developmental expression of dentin sialoprotein (DSP) mRNA.
  • To explore the potential role of DSP in epithelial-mesenchymal interactions during tooth development.

Main Methods:

  • Utilized digoxigenin-labeled (DIG)-DSP and 35S-DSP riboprobes for in situ hybridization.
  • Analyzed DSP mRNA expression in developing mouse and rat molars and incisors at specific developmental stages.

Main Results:

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  • DSP transcripts were localized in both young and mature odontoblasts during predentin formation.
  • DSP mRNA was also transiently expressed by pre-ameloblasts in developing teeth.
  • No DSP mRNA was detected in dental pulp, enamel, or other non-dental tissues.

    • Conclusions:

    • Odontoblasts are the primary source of DSP during dentinogenesis.
    • Transient DSP expression in pre-ameloblasts suggests involvement in crucial epithelial-mesenchymal signaling pathways.
    • These findings provide new insights into the complex molecular regulation of tooth development.