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Updated: Aug 2, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Rapid direct diagnosis of mumps meningitis by ELISA capture technique
J J Chomel1, Y Robin, R Durdilly
1CHU de Lyon, laboratoire de virologie, Faculté de médecine, France.
Abstract:
ELISA capture technique (ELISAc) was carried out using a rabbit hyperimmune serum attached to a solid phase for capturing mumps antigens in cerebrospinal fluid (CSF) in patients with meningitis and/or in supernatants of infected Vero cells. A biotin-labelled rabbit serum prepared from the previous serum was added and the reaction was read by an enzymatic (avidine-peroxidase) reaction by automated reading. The cut-off was calculated in 100 CSFs negative for viruses by conventional diagnosis. The specificity was evaluated in Vero cells infected with 22 CSFs collected from vaccinated children (URABE AM9 attenuated vaccine) who developed meningitis. A guinea pig hyperimmune serum confirmed the specificity. Results in culture correlated with the ELISA capture technique (ELISAc). No cross-reactivity was observed with parainfluenza 1, 2, 3 human reference strains. At least 2.5 ngs of purified mumps proteins were detected corresponding to 10(1.5) infectious particles per ml. ELISAc applied directly to 14 CSFs collected from unvaccinated children with meningitis diagnosed five positive cases, whereas in four cases conventional diagnosis had to be undertaken twice. ELISAc permitted the diagnosis of one additional patient. The test can be carried out in 3 h.
Insights
This study introduces a rapid ELISA capture technique (ELISAc) for detecting mumps virus in cerebrospinal fluid (CSF). The assay provides accurate mumps diagnosis in just 3 hours, improving patient outcomes.
Area of Science:
- Virology
- Immunology
- Diagnostic Medicine
Background:
- Mumps virus infection can cause meningitis, a serious neurological condition.
- Accurate and rapid diagnosis of mumps meningitis is crucial for timely treatment and management.
- Conventional diagnostic methods for mumps virus can be time-consuming and may require multiple tests.
Purpose of the Study:
- To develop and evaluate a rapid ELISA capture technique (ELISAc) for detecting mumps virus antigens.
- To assess the sensitivity and specificity of the ELISAc for diagnosing mumps meningitis in cerebrospinal fluid (CSF).
- To compare the performance of ELISAc with conventional diagnostic methods.
Main Methods:
- ELISA capture technique (ELISAc) utilizing rabbit hyperimmune serum for mumps antigen capture from CSF and infected cell supernatants.
- Biotin-labeled secondary antibody and an avidin-peroxidase enzymatic reaction for detection.
- Specificity confirmed using CSF from vaccinated children and cross-reactivity testing with other human parainfluenza viruses.
- Cut-off value determined using 100 virus-negative CSF samples.
Main Results:
- The ELISAc detected mumps antigens with high specificity, showing no cross-reactivity with common parainfluenza strains.
- Sensitivity was established at 2.5 ng of purified mumps proteins, equivalent to 10(1.5) infectious particles/ml.
- In unvaccinated children with meningitis, ELISAc identified 5 positive cases from 14 CSF samples, while conventional methods required re-testing in 4 cases.
- ELISAc enabled the diagnosis of one additional patient compared to conventional methods.
- The entire test procedure can be completed within 3 hours.
Conclusions:
- The developed ELISA capture technique (ELISAc) is a rapid and specific method for diagnosing mumps meningitis.
- ELISAc offers improved diagnostic efficiency, particularly in cases where conventional methods are inconclusive or require repeat testing.
- This assay has the potential to significantly shorten the time to diagnosis for mumps virus infections.
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