[Effect of nicotine-administration on tyrosine hydroxylase-containing neuron in the rat forebrain;...

Nicotine is toxic substance that is absorbed by cigarette smoking and easily causes dependence. It is clear that smoking is bad for health, however, the effects of nicotine itself on the central nervous system have not been elucidated. We studied the effects of nicotine-administration (5 mg/kg x 2/day, 7 days) on immunoreactivity for tyrosine hydroxylase (TH), the rate-limiting enzyme of catecholamine synthesis, in the rat forebrain including the cerebral cortex, hippocampus, striatum and hypothalamus to investigate the influence on catecholaminergic neurons. In the nicotine-administration group, both the number and intensity of TH-immunoreactive fibers and terminals increased in the fronto-parietal cortex, anterior cingulate cortex and hippocampus in comparison with those of the control group, and a significant difference was demonstrated by computer assisted morphometric analysis. These findings suggest that nicotine-administration influences catecholaminergic neural systems in the forebrain, especially in the cerebral cortex and hippocampus and that these effects might be related to developing the dependence on smoking.