Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Introduction to diagnostic laboratory immunology

T A Fleisher, R H Tomar

    JAMA
    |December 13, 1997
    PubMed
    Summary

    Diagnostic immunology assays utilize antigen-antibody reactions for disease diagnosis. Key methods include flow cytometry and molecular techniques for conditions like hematologic malignancies and immunodeficiencies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A 20 Year Clinical and Laboratory Study of Familial B-Chronic Lymphocytic Leukemia in a Single Kindred.

    Leukemia & lymphoma·2016
    Same author

    Loss of B Cells in Patients with Heterozygous Mutations in IKAROS.

    The New England journal of medicine·2016
    Same author

    Activated STING in a vascular and pulmonary syndrome.

    The New England journal of medicine·2014
    Same author

    The relationship between alloimmunization and posttransfusion granulocyte survival: experience in a chronic granulomatous disease cohort.

    Transfusion·2010
    Same author

    Application of flow cytometry in the assessment of lymphoid reconstitution following BM transplantation.

    Cytotherapy·2010
    Same author

    Isotype determination of antibodies.

    Current protocols in immunology·2008

    Area of Science:

    • Immunology
    • Clinical Diagnostics
    • Laboratory Medicine

    Background:

    • Diagnostic immunology laboratories are crucial for managing diverse clinical conditions.
    • Assays primarily rely on antigen-antibody reactions.
    • Understanding immunologic principles is key to accurate laboratory testing.

    Purpose of the Study:

    • To review fundamental immunologic principles in diagnostic laboratory assays.
    • To highlight specific applications of advanced techniques in clinical practice.
    • To provide an overview of current diagnostic immunology methodologies.

    Main Methods:

    • Review of established principles of antigen-antibody reactions.
    • Discussion of flow cytometric analyses.
    • Explanation of protein electrophoretic assays.
    • Overview of molecular techniques.

    Main Results:

    • Flow cytometry is critical for diagnosing hematologic malignancies, acquired immunodeficiency syndrome (AIDS), and monitoring transplant patients.
    • Protein electrophoresis aids in identifying monoclonal gammopathies.
    • Molecular techniques are vital for diagnosing hematologic malignancies and primary immunodeficiencies.
    • Major histocompatibility complex (MHC) characterization and viral burden enumeration are discussed.

    Conclusions:

    • Diagnostic immunology assays are essential for patient care across various conditions.
    • A range of techniques, from antigen-antibody reactions to molecular methods, are employed.
    • These assays support diagnosis, management, and monitoring of complex diseases.

    Related Experiment Videos