Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human endothelin-converting enzyme (ECE-1): three isoforms with distinct subcellular localizations

A Schweizer1, O Valdenaire, P Nelböck

  • 1F. Hoffmann-La Roche Ltd., Pharma Division, Preclinical Research, Grenzacherstrasse 124, CH-4070 Basel, Switzerland.

The Biochemical Journal
|February 7, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility of ultrasound-based measurement of dorsal scaphoid displacement: A 3D-model study.

Hand surgery & rehabilitation·2021
Same author

Triquetrum osteotomy to approach an otherwise inaccessible coronal malunion of the hamate.

Hand surgery & rehabilitation·2021
Same author

Primarily conservative treatment for triple (A2-A3-A4) finger flexor tendon pulley disruption.

Hand surgery & rehabilitation·2021
Same author

Computer-assisted open reduction internal fixation of intraarticular radius fractures navigated with patient-specific instrumentation, a prospective case series.

Archives of orthopaedic and trauma surgery·2021
Same author

Recent advances in the surgical treatment of malunions in hand and forearm using three-dimensional planning and patient-specific instruments.

Hand surgery & rehabilitation·2020
Same author

[Sport climbing, bouldering and associated injuries in childhood and adolescence].

Der Orthopade·2019

Researchers discovered a third human endothelin-converting enzyme 1 (ECE-1) isoform, ECE-1c, bringing the total to three. This new isoform, along with ECE-1a and ECE-1b, exhibits distinct cellular locations and similar enzymatic activity in converting big endothelins.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Endothelin-converting enzyme 1 (ECE-1) is a key metalloprotease in endothelin synthesis.
  • Previously, two human ECE-1 isoforms (ECE-1a and ECE-1b) were identified.

Purpose of the Study:

  • To identify and characterize novel human ECE-1 isoforms.
  • To investigate the differences and similarities among ECE-1 isoforms.

Main Methods:

  • Cloning of the novel ECE-1c isoform.
  • Ribonuclease protection assays to analyze mRNA expression.
  • Immunofluorescence microscopy to determine subcellular localization.
  • Enzymatic assays to assess kinetic properties.

Main Results:

Related Experiment Videos

  • A third human ECE-1 isoform, ECE-1c, was identified, originating from a single gene via alternative promoters.
  • ECE-1c mRNA is the predominant isoform in most human tissues.
  • ECE-1a and ECE-1c localize to the cell surface, while ECE-1b is found in the trans-Golgi network.
  • All three isoforms display similar kinetic parameters for big endothelin processing.

Conclusions:

  • The existence of three distinct human ECE-1 isoforms (ECE-1a, ECE-1b, and ECE-1c) has been established.
  • Isoform-specific subcellular localization suggests differential roles in endothelin regulation.
  • Despite localization differences, the catalytic efficiency of the isoforms for big endothelin conversion is comparable.