Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electric field directed nucleic acid hybridization on microchips

C F Edman1, D E Raymond, D J Wu

  • 1Nanogen, 10398 Pacific Center Court, San Diego, CA 92121, USA.

Nucleic Acids Research
|February 28, 1998
PubMed
Summary

Optimizing physical parameters like current and voltage on microelectronic arrays accelerates DNA hybridization. Specific zwitterionic buffers, such as histidine, enhance DNA concentration and hybridization, unlike others like glycine.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A multicenter study on the classification and repair effects of stage 4 pressure injury in ischial tuberosity in elderly patients].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025
Same author

[Proliferation inhibition effects of matrine on natural killer/T-cell lymphoma cells mediated by c-Myc signaling pathway].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2020
Same author

The Rorschach butterfly, understanding bone biomechanics prior to using nomenclature in bone trauma interpretations.

Forensic science international·2019
Same author

[Experimental study on DEHP affect the neurodevelopment through interfering with placental thyroid hormones transport].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2018
Same author

Shortened therapy of eight weeks with paritaprevir/ritonavir/ombitasvir and dasabuvir is highly effective in people with recent HCV genotype 1 infection.

Journal of viral hepatitis·2018
Same author

Week 96 results of the randomized, multicentre Maraviroc Switch (MARCH) study.

HIV medicine·2017

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Biology

Background:

  • Microelectronic arrays offer platforms for DNA manipulation.
  • Controlling DNA transport and hybridization is crucial for molecular diagnostics and assays.

Purpose of the Study:

  • To investigate the impact of physical parameters and buffer species on DNA transport, concentration, and hybridization on microelectronic arrays.
  • To identify specific buffer conditions that optimize DNA hybridization reactions.

Main Methods:

  • Utilized active microelectronic arrays to control DNA manipulation.
  • Adjusted physical parameters including DC current, voltage, solution conductivity, and buffer species.
  • Analyzed DNA transport, site-specific concentration, and hybridization efficiency under various conditions.

Related Experiment Videos

Main Results:

  • DNA transport is generally inversely proportional to buffer conductivity.
  • Zwitterionic and low-conductivity buffers (histidine, imidazole, pyridine, collidine) promoted rapid DNA transport, site-specific concentration, and accelerated hybridization.
  • Buffers like glycine and beta-alanine facilitated transport and concentration but not hybridization.

Conclusions:

  • Specific buffer species, particularly zwitterionic ones like histidine, are essential for efficient DNA hybridization on microelectronic arrays.
  • The effectiveness of these buffers is linked to their ability to concentrate DNA via electric fields, buffer anode conditions, and reduce DNA strand repulsion.