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Multiple nucleosome positioning with unique rotational phasing on multimers of the light-responsive elements of pea
R Alessi1, S Cacchione, P De Santis
1Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Moleculare, Università degli Studi di Roma La Sapienza, Italy.
Abstract:
Nucleosome positioning along two DNA tracts, corresponding to tetramers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes, were studied by experimental (exonuclease III mapping and band shift electrophoresis) as well as theoretical methods. Multiple nucleosome positioning with unique rotational phase was derived from both methods in satisfactorily good agreement, if nucleosome dyad axis positions are considered. Theoretical and experimental distributions of nucleosome frequencies appear different, probably on account of DNA sequence dependent digestion kinetics of exonuclease III.