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Related Experiment Videos

Different cells and cell lines produce factors that modulate Sertoli cell function

E Hoeben1, L Deboel, L Rombauts

  • 1Laboratory for Experimental Medicine and Endocrinology, Onderwijs en Navorsing, Gasthuisberg, Catholic University of Leuven, Belgium.

Molecular and Cellular Endocrinology
|May 1, 1994
PubMed
Summary

Factors modulating Sertoli cell function (P-Mod-S) are produced by various cell lines, not just peritubular myoid cells. These P-Mod-S-like factors appear to be similar in size across different cell types.

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Endocrinology

Background:

  • Peritubular myoid cells from rat testes secrete factors (P-Mod-S) that influence Sertoli cell function.
  • Prostatic stromal cells share similarities with peritubular myoid cells and produce analogous mediators.
  • Androgens regulate the secretion of these P-Mod-S factors.

Purpose of the Study:

  • To investigate if myoid cell lines can serve as a source of P-Mod-S-like factors.
  • To compare the activity of conditioned media from myoid and non-myoid cell lines on Sertoli cell function.
  • To characterize the nature of the active principles responsible for modulating Sertoli cell function.

Main Methods:

  • Conditioned media from various cell lines (myoid, fibroblasts) were used to treat Sertoli cells.

Related Experiment Videos

  • Sertoli cell transferrin secretion was measured as an endpoint for P-Mod-S-like activity.
  • Chromatographic techniques (Superdex 75, reversed-phase) were employed to assess factor size.

    • Main Results:

    • Conditioned media from all tested cell lines, including fibroblasts, modulated Sertoli cell function similarly to peritubular and stromal cell media.
    • The observed activity was not due to fetal calf serum contamination or classical growth factors.
    • Factors from different cell origins exhibited similar molecular sizes (45-50 kDa).

    Conclusions:

    • Mediators with P-Mod-S-like activity are produced by diverse cell types and cell lines, irrespective of smooth muscle characteristics.
    • The active principles responsible for modulating Sertoli cell function appear conserved across various cell origins.
    • Further research is needed to confirm the identity of these active principles.