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Induction of thioredoxin in human lymphocytes with low-dose ionizing radiation
Y Hoshi1, H Tanooka, K Miyazaki
1Nuclear Energy Systems Department, Central Research Institute of Electric Power Industry, Komae-shi, Tokyo, Japan. hoshi@criepi.denken.or.jp
Abstract:
Induction of the expression of the thioredoxin (TRX) gene, producing a key protein in regulating cellular functions through redox reaction as well as being a radioprotector, was followed after ionizing irradiation of lymphocytes from human donors. The TRX mRNA level increased to a peak, 5.7-fold higher than the control at maximum, 6 h after irradiation, and then decreased. The optimum radiation dose for enhancement of induction of the TRX mRNA was 0.25 Gy. The TRX protein also increased to a peak, a 3-fold increase at maximum, with the same timing as that for TRX mRNA.