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Multicenter evaluation of the updated and extended API (RAPID) Coryne database 2.0
G Funke1, F N Renaud, J Freney
1Department of Medical Microbiology, University of Zürich, Switzerland. funke@immv.unizh.ch
Journal of Clinical Microbiology
|December 17, 1997
Summary
The updated API (RAPID) Coryne system with database 2.0 effectively identifies coryneform bacteria, achieving 90.5% accuracy. While requiring additional tests for some strains, it remains a valuable tool for clinical laboratories.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Bacterial Identification
Background:
- Coryneform bacteria represent a diverse group of microorganisms frequently encountered in clinical settings.
- Accurate identification of coryneform bacteria is crucial for appropriate patient management and infection control.
- Previous versions of the API (RAPID) Coryne system have been used for bacterial identification, necessitating updates to reflect taxonomic changes and new species.
Purpose of the Study:
- To evaluate the performance and accuracy of the updated API (RAPID) Coryne system with database 2.0 for identifying coryneform bacteria.
- To assess the system's capability in correctly identifying a broad range of coryneform taxa, including those newly described or reclassified.
- To compare the identification efficiency of database 2.0 with previous versions, particularly regarding the need for supplementary tests.
Main Methods:
- A multicenter study involving 407 strains of coryneform bacteria.
- Utilized the API (RAPID) Coryne system with database 2.0, featuring 20 biochemical reactions.
- Included strains belonging to taxa covered by database 2.0 and those not covered, alongside additional differential tests.
Main Results:
- The system correctly identified 90.5% of strains within the taxa covered by database 2.0.
- Additional tests were required for 55.1% of all strains for complete identification.
- A low rate of unidentified (5.6%) and misidentified (3.8%) strains was observed, with specific challenges noted for Corynebacterium coyleae, Propionibacterium acnes, and Aureobacterium spp.
Conclusions:
- The API (RAPID) Coryne system 2.0 demonstrates utility in identifying diverse coryneform bacteria in routine clinical laboratories.
- Despite an increased need for supplementary tests due to taxonomic updates, the system provides reliable identification.
- The updated database accommodates recent taxonomic shifts and newly identified organisms, enhancing its diagnostic value.