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Reproducibility of AMPLICOR enterovirus PCR test results

H A Rotbart1

  • 1University of Colorado Health Sciences Center, Denver 80262, USA.

Journal of Clinical Microbiology
|December 17, 1997
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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