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Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis
G Prod'hom1, C Guilhot, M C Gutierrez
1Centre National de Référence des Mycobactéries, Institut Pasteur, Paris, France.
Journal of Clinical Microbiology
|December 17, 1997
Abstract:
A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.