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Histone Sequence Database: new histone fold family members
1Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Nucleic Acids Research
|February 21, 1998
Summary
A new histone protein database offers over 1000 annotated sequences, including histone fold family members like human DRAP1. This resource aids researchers by providing alignments, structural data, and conflict information for histone proteins.
Area of Science:
- Molecular Biology
- Bioinformatics
- Genomics
Background:
- Histones are crucial proteins involved in DNA packaging and gene regulation.
- Existing databases may lack comprehensive, annotated collections of histone and histone fold family sequences.
Purpose of the Study:
- To create a comprehensive, annotated database of histone and histone fold family protein sequences.
- To integrate diverse data, including sequence alignments and structural information, for enhanced research accessibility.
Main Methods:
- Searched public protein databases using core and linker histone sequences from chicken and humans.
- Employed two distinct motif search algorithms to identify histone fold family members.
- Compiled annotated sequences, multiple sequence alignments, and structural data.
Main Results:
- Developed an annotated database of over 1000 histone protein sequences.
- Identified novel histone fold family members, such as human DRAP1 and yeast CSE4.
- Integrated features like cross-database conflict information, Entrez links, and Cn3D visualization.
Conclusions:
- The new database provides a valuable, searchable resource for researchers studying histones and histone fold proteins.
- Enhanced data integration facilitates comparative genomics, structural biology, and functional studies.
- The database is publicly accessible via the World Wide Web.