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Maintenance and down-regulation of primed neutrophil chemiluminescence activity in human whole blood
G E Brown1, J Reiff, R C Allen
1Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
Abstract:
Priming of polymorphonuclear neutrophils (PMN) in whole blood (by tumor necrosis factor alpha and interleukin-8 for enhancement of luminol-dependent chemiluminescence induced by human complement-opsonized zymosan) was stable for 120 min. In contrast, priming of isolated PMN in plasma-free suspension for responses to opsonized zymosan, formyl-methionyl-leucyl-phenylalanine, and phorbol myristate acetate was markedly less stable. Decay of priming was not due to irreversible inactivation of the terminal CL production machinery because PMN could be reprimed by platelet-activating factor or leukotriene B4. The tumor necrosis factor-alpha-primed state of isolated PMN was stabilized by host plasma in a concentration-dependent fashion. We conclude that PMN priming results in a dynamic state that is reversible. Our findings suggest the existence of blood-borne components that may act to stabilize or modify PMN priming.
Insights
Neutrophil priming in whole blood is stable, but priming of isolated neutrophils is not. Blood components stabilize neutrophil priming, suggesting a dynamic and reversible state.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Neutrophil priming enhances their response to stimuli.
- The stability of this primed state is crucial for understanding neutrophil function in vivo.
Purpose of the Study:
- To investigate the stability of neutrophil priming in different conditions.
- To identify factors influencing the duration and reversibility of neutrophil priming.
Main Methods:
- Priming polymorphonuclear neutrophils (PMN) in whole blood and isolated suspensions.
- Assessing luminol-dependent chemiluminescence responses to various stimuli.
- Evaluating the effect of plasma and other factors on PMN priming stability.
Main Results:
- Priming of PMN in whole blood remained stable for 120 minutes.
- Priming of isolated PMN was significantly less stable.
- Decay of priming was reversible, as PMN could be re-primed.
- Host plasma stabilized tumor necrosis factor-alpha-primed isolated PMN.
Conclusions:
- Neutrophil priming represents a dynamic and reversible state.
- Blood-borne factors likely play a role in stabilizing or modulating neutrophil priming.