Inhibition of the metallo-beta-lactamase produced from Serratia marcescens by thiol compounds
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M Goto1, T Takahashi, F Yamashita
1Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Biological & Pharmaceutical Bulletin
|December 24, 1997
Abstract:
Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-beta-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with Ki of 0.23 and 0.19 microM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.