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Protease-induced multicell formation in Staphylococcus haemolyticus

K Yabu1, Y Nishiyama, T Ochiai

  • 1Faculty of Pharmaceutical Science, Hokuriku University, Kanazawa, Ishikawa, Japan.

Microbiology and Immunology
|January 1, 1997
PubMed
Summary

Protease addition to Staphylococcus haemolyticus cultures induced multicellular tetrads by inhibiting cell wall separation. These multicells showed increased colony-forming units, suggesting potential applications in bacterial cultivation.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Genetics

Background:

  • Staphylococcus haemolyticus is a common human commensal and opportunistic pathogen.
  • Bacterial cell division and separation are critical processes influencing colony formation and biofilm development.

Purpose of the Study:

  • To investigate the effect of protease on the multicellularity of Staphylococcus haemolyticus.
  • To elucidate the mechanism underlying protease-induced multicellular formation.

Main Methods:

  • Induction of multicellularity using protease in exponentially growing cultures.
  • Electron microscopy for visualizing cell morphology.
  • Electrophoretic analysis to assess cell wall-lytic activity.

Main Results:

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  • Protease treatment efficiently induced tetrad-shaped multicellular structures in Staphylococcus haemolyticus.
  • Multicells were septated but remained attached.
  • A fourfold increase in colony-forming units was observed when multicells were incubated with specific extracts.
  • Protease treatment led to a loss of cell wall-lytic activity.

Conclusions:

  • Protease inhibits cross-wall separation, leading to the formation of Staphylococcus haemolyticus multicells.
  • The observed increase in colony-forming units suggests potential for enhanced bacterial cultivation using this method.