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Amylose-iodine complex. II. Molecular weight estimates

F R Dintzis, R Tobin, A C Beckwith

    Macromolecules
    |May 1, 1976
    PubMed
    Summary

    Ultracentrifugation reveals that amylose-iodine-iodide (AI) complexes exhibit significant heterogeneity and aggregation. Molecular weights of AI complexes increase with potassium iodide concentration and over time.

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    Area of Science:

    • Macromolecular science
    • Biophysical chemistry
    • Polymer physics

    Background:

    • Amylose-iodine-iodide (AI) complexes are crucial in understanding carbohydrate-iodine interactions.
    • Previous studies indicated complex behavior of AI complexes in solution.

    Purpose of the Study:

    • To investigate the molecular weight and solution behavior of amylose-iodine-iodide (AI) complexes using ultracentrifugation.
    • To characterize the heterogeneity and aggregation of AI complexes.

    Main Methods:

    • Archibald method for ultracentrifugation.
    • Sedimentation equilibrium measurements.
    • Extrapolation of molecular weight data.

    Main Results:

    • Apparent molecular weights of AI complexes were determined at various conditions.
    • Significant heterogeneity and aggregation were observed in AI complex solutions.
    • Apparent molecular weights increased with potassium iodide concentration and over time.
    • Molecular weight estimates were consistent across different iodine-binding saturation levels.

    Conclusions:

    • AI complex solutions are characterized by heterogeneity and aggregation.
    • Solution behavior, including apparent molecular weight, is sensitive to potassium iodide concentration and time.
    • These findings provide insights into the physical chemistry of AI complexes.

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