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Perflubron decreases inflammatory cytokine production by human alveolar macrophages
M J Thomassen1, L T Buhrow, H P Wiedemann
1Department of Pulmonary and Critical Care Medicine, The Cleveland Clinic Foundation, OH 44195-5038, USA.
Objective:
To determine whether inflammatory cytokine production by stimulated human alveolar macrophages is affected by perflubron exposure.
Design:
Controlled laboratory investigation of alveolar macrophage function in vitro.
Setting:
Research laboratory.
Subjects:
Cultured alveolar macrophages obtained by bronchoalveolar lavage from eleven normal volunteers.
Interventions:
Endotoxin-stimulated alveolar macrophages were treated with perflubron.
Measurements And Main Results:
Alveolar macrophages were stimulated for 1 hr with lipopolysaccharide and then treated with perflubron for 23 hrs. Cell-free supernatants were collected and cytokines were assayed by enzyme-linked immunosorbent assay. Tumor necrosis factor-alpha, interleukin-1, and interleukin-6 were stimulated by lipopolysaccharide (endotoxin) and all of these cytokines were significantly (p < .05) inhibited by perflubron. Cell viability was not affected by perflubron. Basal cytokine concentrations from unstimulated alveolar macrophages were not altered by perflubron.
Conclusions:
Exposure of stimulated human alveolar macrophages to perflubron in vitro decreases cytokine production. This observation suggests that perflubron may have anti-inflammatory activity.
Insights
Perflubron exposure significantly reduced inflammatory cytokine production in stimulated human alveolar macrophages. This suggests perflubron may possess anti-inflammatory properties without affecting cell viability.
Area of Science:
- Immunology
- Cell Biology
- Pharmacology
Background:
- Alveolar macrophages play a crucial role in lung immune responses.
- Inflammatory cytokines mediate various lung pathologies.
- Perflubron is a fluorocarbon liquid with potential therapeutic applications.
Purpose of the Study:
- To investigate the effect of perflubron on inflammatory cytokine production by human alveolar macrophages.
- To determine if perflubron influences the function of stimulated macrophages.
Main Methods:
- Human alveolar macrophages were obtained from healthy volunteers via bronchoalveolar lavage.
- Macrophages were stimulated with lipopolysaccharide (endotoxin).
- Stimulated macrophages were treated with perflubron, and cytokine levels in supernatants were measured using ELISA.
Main Results:
- Lipopolysaccharide stimulation significantly increased tumor necrosis factor-alpha, interleukin-1, and interleukin-6 production.
- Perflubron treatment significantly inhibited the production of these cytokines (p < .05).
- Perflubron did not affect the viability of alveolar macrophages or basal cytokine levels.
Conclusions:
- Perflubron exposure in vitro reduces inflammatory cytokine release from stimulated human alveolar macrophages.
- These findings suggest that perflubron may possess anti-inflammatory activity in the lung.
- Further research is warranted to explore the therapeutic potential of perflubron in inflammatory lung diseases.