Dynamics of haemostasis during the oestrous cycle and pregnancy in bitches

The blood coagulation status was studied in 31 bitches of different breeds during 33 oestrous cycles and during nine pregnancies. Two other bitches were ovariohysterectomized and received subcutaneous injections of oestradiol benzoate for 7 consecutive days. Blood samples were taken in early and late follicular phases, at ovulation, at day 1 after the end of oestrus as determined by cytology, at days 30, 60, 90 and 120 of metoestrus and in anoestrus. The samples were analysed for the concentrations of fibrinogen, fibrin(ogen) degradation products, as well as for the prothrombin time, the activated partial thromboplastin time, the antithrombin III activity, the number of platelets and the haematocrit. In other blood plasma samples the concentrations of oestradiol and progesterone were measured. In the two bitches that were ovariohysterectomized and received subcutaneous injections of oestradiol benzoate for 7 consecutive days, the coagulation parameters and hormones were examined in blood samples collected at appropriate terms and time intervals as in intact dogs. The significantly increased concentrations of fibrinogen and fibrin(ogen) degradation products, the large number of platelets and the decreased antithrombin III activity observed during the luteal phase of the nonpregnant and pregnant bitches are attributed to direct or indirect effects of the high peripheral progesterone concentrations. In the mid-luteal phase (day 30) this activation was more distinct during pregnancy than in the nonpregnant dogs presumably owing to additional effects of local processes in the uteroplacental area. Influences of high concentrations of oestradiol were not observed either during the follicular phase of the intact bitches or after oestradiol benzoate administration in the ovariohysterectomized dogs.