Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vinculin is associated with the E-cadherin adhesion complex

R B Hazan1, L Kang, S Roe

  • 1Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.

The Journal of Biological Chemistry
|January 24, 1998
PubMed
Summary

Vinculin can mediate E-cadherin adhesion in breast cancer cells lacking alpha-catenin by binding to beta-catenin. This suggests vinculin plays a role in cell adhesion complex regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Current status and advances in the management of rectal anastomotic stenosis].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2026
Same author

SACI-IO HR+: A randomized phase II trial of sacituzumab govitecan with or without pembrolizumab in patients with metastatic hormone receptor-positive/HER2-negative breast cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Caplan syndrome presenting as tumour-like pulmonary nodules in a patient with rheumatoid arthritis: a diagnostic challenge.

Scandinavian journal of rheumatology·2026

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Cadherins are crucial for calcium-dependent cell-cell adhesion.
  • Alpha-catenin links cadherins to the cytoskeleton, and its absence causes non-adhesion.
  • E-cadherin expression and adhesion are modulated by cellular conditions.

Purpose of the Study:

  • To investigate E-cadherin-mediated adhesion in breast cancer cells lacking alpha-catenin.
  • To identify alternative mechanisms regulating cadherin-based cell adhesion.
  • To explore the role of vinculin in E-cadherin adhesion complexes.

Main Methods:

  • Utilized MDA-MB-468 breast cancer cells with alpha-catenin mutations.
  • Performed serum starvation to induce E-cadherin expression and adhesion.

Related Experiment Videos

  • Employed coprecipitation and immunoprecipitation assays to analyze protein interactions.
  • Used recombinant proteins to test binding competition.

    • Main Results:

    • Serum starvation induced E-cadherin adhesion in MDA-MB-468 cells despite absent alpha-catenin.
    • Vinculin was found associated with E-cadherin complexes in these cells.
    • Both alpha-catenin and vinculin can interact with beta-catenin, suggesting competitive binding.
    • E-cadherin and vinculin showed strong association in MDA-MB-468 cells.

    Conclusions:

    • Vinculin can mediate E-cadherin-dependent cell adhesion in the absence of alpha-catenin.
    • Vinculin likely interacts with beta-catenin, suggesting a role in regulating cadherin-based adhesion complexes.
    • This highlights an alternative pathway for establishing cell adhesion in cancer cells.