Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrin expression in developing smooth muscle cells

R Wang1, M H Stromer, T W Huiatt

  • 1Muscle Biology Group, Department of Animal Science and Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011-3260, USA.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|December 24, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiology of ghrelin and related peptides.

Domestic animal endocrinology·2005
Same author

Action of free radical in podophyllic acid piperindyl hydrazone nitroxide radical on its antitumor activity and toxicity.

Zhongguo yao li xue bao = Acta pharmacologica Sinica·2000
Same author

Amiodarone induces apoptosis of human and rat alveolar epithelial cells in vitro.

American journal of physiology. Lung cellular and molecular physiology·2000
Same author

Cadmium and iron transport by members of a plant metal transporter family in Arabidopsis with homology to Nramp genes.

Proceedings of the National Academy of Sciences of the United States of America·2000
Same author

Neurokinin A, calcitonin gene-related peptide, and dynorphin A (1-8) in spinal dorsal horn contribute to descending inhibition evoked by nociceptive afferent pathways: an immunocytochemical study.

Regulatory peptides·2000
Same author

Cell loss in isolated human islets occurs by apoptosis.

Pancreas·2000
Same journal

Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Aquaporin-4 Localization in Terminal Glial Cells of Human Mechanosensory Corpuscles: An Immunohistochemical Study of Penile Tissues.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
See all related articles

Alpha1 and beta1 integrins, key cell adhesion receptors, increase in avian gizzard smooth muscle during development. Their expression is synchronous and localized to dense plaques, coinciding with muscle development.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Integrins are crucial cell adhesion receptors in smooth muscle.
  • Understanding integrin expression during development is vital for muscle formation.

Purpose of the Study:

  • To investigate the expression patterns and distribution of alpha1 and beta1 integrin subunits in developing avian smooth muscle.
  • To determine if alpha1 and beta1 integrin expression is sequential or synchronous during development.

Main Methods:

  • SDS-PAGE and immunoblotting to quantify integrin levels.
  • Immunoelectron microscopy to visualize integrin distribution.
  • Analysis of avian gizzard smooth muscle from embryonic to post-hatch stages.

Main Results:

Related Experiment Videos

  • Alpha1 and beta1 integrin levels increase with age, with significant rises post-hatch.
  • Statistically significant increases in integrin labeling observed at key developmental time points.
  • Integrins prominently localize to membrane-associated dense plaques and filament anchoring regions.

Conclusions:

  • Alpha1 and beta1 integrin expression is synchronous during smooth muscle development.
  • Integrin expression correlates with intracellular proliferation and myofilament reorientation.
  • These findings highlight the coordinated role of integrins in avian gizzard smooth muscle development.