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Chemiluminescence response of whole blood in patients undergoing urological operations
M Sakumoto1, T Matsumoto, O Mochida
1Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Abstract:
Polymorphonuclear leukocytes (PMNs) are one of the most important components of the defence mechanisms against bacterial infection. The functions of PMNs are believed to be impaired in patients during the perioperative period. Bactericidal function of PMNs was investigated together with the luminol-dependent chemiluminescence (CL) reaction of whole blood in 23 patients, 12 undergoing open surgery and 11 undergoing endoscopic surgery. Blood samples were collected one day before surgery (day -1) and 2 hours (day 0), 24 hours (day 1) and 7 days (day 7) after surgery. Counts of whole white blood cells (WBCs), PMNs and lymphocytes were not different between the two surgery groups. CL responses in the open surgery group were increased on days 0, 1 and 7. In the endoscopic surgery group, CL response was increased on day 1, but not on day 0 or day 7. These results suggest that the PMN function during the perioperative period was not impaired, but increased just after surgery, mainly due to an increasing number of WBC caused by the surgical intervention.
Insights
Perioperative polymorphonuclear leukocyte (PMN) function, crucial for fighting bacterial infections, was assessed. Results indicate PMN function increases post-surgery, contrary to prior beliefs of impairment.
Area of Science:
- Immunology
- Surgical Science
- Infectious Disease
Background:
- Polymorphonuclear leukocytes (PMNs) are vital for combating bacterial infections.
- PMN function is often presumed to be compromised during the perioperative period.
- Understanding immune responses to surgery is critical for patient outcomes.
Purpose of the Study:
- To investigate the bactericidal function and luminol-dependent chemiluminescence (CL) of PMNs in patients undergoing surgery.
- To compare immune responses between open surgery and endoscopic surgery.
- To determine if PMN function is impaired or altered in the perioperative period.
Main Methods:
- Assessed luminol-dependent chemiluminescence (CL) of whole blood in 23 patients.
- Compared open surgery (12 patients) and endoscopic surgery (11 patients).
- Collected blood samples pre-surgery (day -1) and post-surgery (days 0, 1, and 7).
Main Results:
- No significant differences in white blood cell (WBC), PMN, or lymphocyte counts between surgery types.
- Open surgery group showed increased CL responses on days 0, 1, and 7 post-surgery.
- Endoscopic surgery group exhibited increased CL response only on day 1 post-surgery.
Conclusions:
- Surgical intervention does not impair, but rather enhances, PMN function in the immediate perioperative period.
- Increased WBC counts post-surgery likely contribute to the observed enhancement in PMN function.
- The type of surgery may influence the duration and magnitude of the PMN response.