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An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR
A Sanyal1, S W O'Driscoll, M E Bolander
1Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
Molecular Biotechnology
|December 24, 1997
Abstract:
A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65 degrees C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.