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The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida
B H Jost1, P Homchampa, R A Strugnell
1Department of Microbiology, Monash University, Clayton VIC, Australia.
Molecular Biotechnology
|December 24, 1997
Summary
The Bacillus subtilis sacB gene was tested in Pasteurella multocida strains. It failed as a positive selection system, as the bacteria did not become sensitive to sucrose.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Pasteurella multocida is a significant pathogen affecting various animal species.
- Genetic manipulation of P. multocida is crucial for understanding its virulence and developing control strategies.
- Counter-selectable markers are valuable tools for genetic engineering in bacteria.
Purpose of the Study:
- To evaluate the efficacy of the Bacillus subtilis sacB gene as a counter-selectable marker in Pasteurella multocida.
- To determine if sacB expression confers sucrose sensitivity in P. multocida serogroups A and B.
Main Methods:
- The sacB gene from Bacillus subtilis was introduced and expressed in Pasteurella multocida strains.
- Sucrose sensitivity was assessed in the engineered P. multocida strains.
Main Results:
- Expression of the sacB gene did not result in sucrose sensitivity in any of the tested Pasteurella multocida strains.
- The sacB gene failed to function as a positive selection system in this bacterial species.
Conclusions:
- The Bacillus subtilis sacB gene is not suitable for use as a positive selection marker in Pasteurella multocida.
- Alternative genetic strategies are required for the manipulation of P. multocida strains.