Related Experiment Videos
The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida
B H Jost1, P Homchampa, R A Strugnell
1Department of Microbiology, Monash University, Clayton VIC, Australia.
Molecular Biotechnology
|December 24, 1997
Abstract:
The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.