Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The structure and functions of the HAP1/Ref-1 protein

D G Rothwell1, G Barzilay, M Gorman

  • 1Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, UK.

Oncology Research
|January 1, 1997
PubMed
Summary

The HAP1 protein has two key roles in cellular defense against oxidative stress: repairing DNA damage and regulating DNA-binding proteins. Its structure-function relationship is crucial for these dual activities.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Health Inequalities Impact Assessment of the surveillance of COVID-19 in asymptomatic patients attending dental settings in Scotland.

Community dental health·2022
Same author

An examination of labor time-use on spring-calving dairy farms in Ireland.

Journal of dairy science·2022
Same author

The Scattered Light Time-history Diagnostic suite at the National Ignition Facility.

The Review of scientific instruments·2021
Same author

Evaluating the association of allergies with multiple sclerosis susceptibility risk and disease activity in a pediatric population.

Journal of the neurological sciences·2017
Same author

Tabletop microsurgical training setup for the price of a pair of loupes.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2017
Same author

Distinct subclonal tumour responses to therapy revealed by circulating cell-free DNA.

Annals of oncology : official journal of the European Society for Medical Oncology·2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • The HAP1/Ref-1 (HAP1) protein is a nuclear enzyme involved in cellular defense against oxidative stress.
  • HAP1 has a well-characterized role in DNA repair of lesions induced by spontaneous hydrolysis or reactive oxygen species (ROS).
  • A second, less understood role involves regulating DNA-binding protein activity through redox modification of cysteine residues.

Purpose of the Study:

  • To review current knowledge on the structure-function relationship of HAP1.
  • To detail the roles of specific amino acid residues in HAP1's catalytic functions.
  • To elucidate the mechanisms underlying HAP1's dual roles in cellular defense.

Main Methods:

  • Literature review of existing studies on HAP1 structure and function.

Related Experiment Videos

  • Analysis of biochemical data on HAP1's DNA repair and protein-binding activities.
  • Examination of structure-activity relationships for key amino acid residues.

    • Main Results:

    • HAP1's dual functions in DNA repair and redox regulation are structurally dependent.
    • Specific amino acid residues are critical for HAP1's catalytic activity in DNA repair.
    • The redox mechanism for HAP1's regulation of DNA-binding proteins is still under investigation.

    Conclusions:

    • HAP1's structure dictates its essential dual roles in maintaining cellular integrity under oxidative stress.
    • Understanding HAP1's catalytic mechanisms can inform strategies for combating oxidative damage.
    • Further research is needed to fully characterize HAP1's redox-dependent regulatory functions.