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One-step purification of nisin A by immunoaffinity chromatography
A M Suárez1, J I Azcona, J M Rodríguez
1Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. anagea@eucmax.sim.ucm.es
Applied and Environmental Microbiology
|December 24, 1997
Abstract:
The lantibiotic nisin A was purified to homogeneity by a single-step immunoaffinity chromatography method. An immunoadsorption matrix was developed by direct binding of anti-nisin A monoclonal antibodies to N-hydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.