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Related Experiment Videos

An avian sodium-hydrogen exchanger

S G Bhartur1, C Bookstein, M W Musch

  • 1Department of Physiology and Biophysics, University of Illinois at Chicago 60612, USA.

Comparative Biochemistry and Physiology. Part A, Physiology
|December 24, 1997
PubMed
Summary

Chicken intestines have novel sodium transporters, suggesting a new avian family of Na+/H+ exchangers (NHEs). This research identifies unique NHE isoforms in birds, distinct from mammalian counterparts.

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Area of Science:

  • Physiology
  • Molecular Biology
  • Comparative Biochemistry

Background:

  • Intestinal sodium transporters, like Na+/H+ exchangers (NHEs), are crucial for sodium conservation in terrestrial birds.
  • Mammals possess at least five NHE isoforms (NHEs 1-5), with distinct cellular localizations.

Purpose of the Study:

  • To investigate the presence and identity of mammalian NHE-1 and NHE-3 isoforms in chicken enterocytes.
  • To characterize the NHE activity in chicken intestinal brush border membranes.

Main Methods:

  • Utilized antisera and cDNA probes against mammalian NHE-1 and NHE-3.
  • Examined protein expression in chicken intestinal brush border and basolateral membranes via Western blotting.
  • Assessed transcript presence using Northern blotting or similar techniques.

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Main Results:

  • Antisera against rat NHE-3 recognized a 97 kDa protein in chicken intestinal brush border membranes.
  • A NHE-3 cDNA probe did not detect any corresponding transcript in chicken intestine.
  • A NHE-1 cDNA probe identified a 3.9 kb transcript, but NHE-1 antibodies did not recognize any protein in chicken membranes.

Conclusions:

  • Chicken intestinal brush border membranes exhibit NHE activity functionally similar to mammalian NHE-3.
  • The study suggests the existence of a novel avian NHE family, distinct from known mammalian isoforms.
  • Multiple NHE isoforms are present in the chicken intestine, with unique characteristics.